Monospecific antibody

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Monospecific antibodies are antibodies that all have affinity for the same antigen. Monoclonal antibodies are monospecific, but monospecific antibodies may also be produced by other means than producing them from a common germ cell.

1 Production
- 1.1 Hybridoma cell
- 1.2 PrEST
2 References
3 See also

[edit] Production

[edit] Hybridoma cell

Main article: Monoclonal antibodies#Production

Monoclonal antibodies are typically made by fusing the spleen cells from a mouse that has been immunized with the desired antigen with myeloma cells. However, recent advances have allowed the use of rabbit B-cells.

[edit] PrEST

Another way of producing monospecific antibodies are by PrESTs. A PrEST (protein epitope signature tag) is a type of recombinantly produced human protein fragment. They are inserted into an animal, e.g. rabbit, which produces antibodies against the fragment. These antibodies are monospecific against the human protein.^[1]

[edit] References

^ Larsson K, Wester K, Nilsson P, Uhlén M, Hober S, Wernérus H (2006). "Multiplexed PrEST immunization for high-throughput affinity proteomics". J. Immunol. Methods 315 (1-2): 110–20. doi:10.1016/j.jim.2006.07.014. PMID 16949094.